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KEMP ET. AL., 2011 MOVIES Click to see Movie 1Click to see Movie 2 Movies showing impaired retrograde transport of endosomes labeled with CTAF488 in axons of sciatic nerve explants from diseased males of two different mouse models of SBMA, a knock-in (KI) model (Movie 1) and a myogenic transgenic (TG) model (Movie 2). Endosomal transport in affected TG males (S1) exhibit a deficit in flux (endosomes/min) compared to WT controls without effects on velocity, whereas KI males (S2) exhibit deficits in both flux and velocity (microns/sec). Time-lapse movies of moving endosomes were made using an inverted confocal microscope in Dr. Miller's lab (Nikon Eclipse TE2000-E, Japan) equipped with a PlanApo 60X oil objective (NA 1.4), 488 nm laser (PraireView, USA) and a Photometrics CoolSNAP HQ2 camera (USA). Each movie is 7 minutes in length, comprising 211 images, with images captured every 2 sec at a 1 sec exposure. Scale bar = 5 μm |